Introduction to HPLC The History of Chromatography; Basic Instrumentation
Basic Principles of HPLC: Theory of Chromatography; Chemical Bonding and Polarity
Mobile Phase Preparation, the Use of Buffers, and Sample Preparation: Mobile Phase Preparation; Buffers; Sample and Standard Preparation
Modes of Separation: Reverse Phase HPLC; Ion Exchange; Normal Phase HPLC
Detection Systems: Theory; UV/Vis Detectors versus Diode Array Detectors; Electrochemical Detectors; Mass Spectrometry
Method Development in Reversed Phase HPLC: Sample Composition; Which Column Will Affect the Separation?; Choosing a Mobile Phase; Automated Method Development; Method Development Strategy; Method Development Worked Example
System Performance; Chromatographic Suitability
Qualification, Validation, and Verification: Valid Analytical Measurement ; Estimation of Uncertainty of Measurement, Sources of Error, and Statistical Evaluation; Instrument Qualification; Method Validation; Method Verification
Quality Systems: What Is Quality?; Introduction to Quality Assurance; How Do We Ensure Quality in Our Laboratories?; A Quality Management System; Accreditation; Auditing
Troubleshooting HPLC Systems: Troubleshooting; Tool Kit and Spare Parts; Preventative Maintenance; Column Care
Forensic Applications of HPLC: Drug Analysis; Toxicological Analysis; Colour Analysis; Analysis of Explosives; Food and the Environment
